post about THC tomatoes
I don’t know if this is any help or not. But to the response of the man who commented on the possibility of injecting a gene that produces THC into tomatoes. You said its probably impossible because of there genetic diversity. I would figure if you knew the amino acid sequence of THC you could manufacture an mRNA strand (the codons that code for a particular amino acid are in reference books and nearly universal for all walks of life). if this strand was read by the RNA polymerase and THC was produced it a good bet that its a useable gene. Unfortunately transformation into the tomato would be VERY difficult because little is known about eukaryotic gene regulation, and what is known is sketchy. I would recommend using bacteria such as E. Coli because transformation is quite easy and genes are by default expressed. As far as producing THC that is useable for human consumption much more is needed. Several attempts at the biological synthesis of a chemical have resulted in consumer disasters. In one experiment I believe they were making tryptophan. They synthesized mRNA and included it into the genome of bacteria. When the levels of tryptophan became extremely high in the cytoplasm the cell made a material called 1-ethylidine-bis-L-tryptophan or EBT. EBT causes horrible side effects in humans. Due to poor lab procedure the tryptophan/EBT concoction was ingested causing many to get sick. The reason the bacteria reverted to producing EBT is still unknown. Moral of the story, anything can be modified to make your desired end product but isolation of the end product is key to safe a manufacturing procedure.
Does anyone have anyone have any notable material on the subject? I am very interested.
I don’t know if this is any help or not. But to the response of the man who commented on the possibility of injecting a gene that produces THC into tomatoes. You said its probably impossible because of there genetic diversity. I would figure if you knew the amino acid sequence of THC you could manufacture an mRNA strand (the codons that code for a particular amino acid are in reference books and nearly universal for all walks of life). if this strand was read by the RNA polymerase and THC was produced it a good bet that its a useable gene. Unfortunately transformation into the tomato would be VERY difficult because little is known about eukaryotic gene regulation, and what is known is sketchy. I would recommend using bacteria such as E. Coli because transformation is quite easy and genes are by default expressed. As far as producing THC that is useable for human consumption much more is needed. Several attempts at the biological synthesis of a chemical have resulted in consumer disasters. In one experiment I believe they were making tryptophan. They synthesized mRNA and included it into the genome of bacteria. When the levels of tryptophan became extremely high in the cytoplasm the cell made a material called 1-ethylidine-bis-L-tryptophan or EBT. EBT causes horrible side effects in humans. Due to poor lab procedure the tryptophan/EBT concoction was ingested causing many to get sick. The reason the bacteria reverted to producing EBT is still unknown. Moral of the story, anything can be modified to make your desired end product but isolation of the end product is key to safe a manufacturing procedure.
Does anyone have anyone have any notable material on the subject? I am very interested.