Plant cell culture

Discussion in 'Biology & Genetics' started by NenarTronian, May 31, 2002.

  1. NenarTronian Teenaged Transhumanist Registered Senior Member

    Messages:
    1,083
    Hello all. A question concerning plant cell cultures, you know, the ones in the nutrient media in the bioreactor. How do these work? I read a little but and i think I get it but still unsure.

    Also, how easy is it to have certain cells of plants (or animals) create a certain substance, such as a chemical or pharmaceutical? For example, a culture of cannabis cells producing THC oils would be a neat and interesting alternative to growing...

    Just out of curiosity! any thoughts would be appreciated.
     
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  3. Pine_net Chaos Product Registered Senior Member

    Messages:
    479
    Are you talking about:

    Sequential Bioreactors?
    Multiphase Bioreactors?
    Solid Waste (MSW) Bioreactors?
    Membrane Bioreactors?

    Tetrahydrocannabinol Extract: Add 50g grass/liter petroleum ether or benzene; soak twelve hours with occasional shaking; filter and extract petroleum ether three times with a solution containing 5% NaOH and 5% Na2SO3. Acidify the aqueous extracts with cold dilute sulfuric acid and extract with ether or chloroform which is dried, evaporated in a vacuum to yield the cannabidiolic acid. THC and cannabidiol remain in the petrolium ether which can be dried and evaporated in a vacuum and the residue added to the grass. THC acid is converted to THC by boiling in benzene for seven hours.

    Or just smoke it... heh heh
     
    Last edited: Jun 5, 2002
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