I am making a eukaryotic cell from scratch. I have gotten to the point of doing PCR of the NUP35 gene. Do I need an amplicon or can I do it without that if the primers are complementary to the start and end of the gene?Please Register or Log in to view the hidden image!
The chromosomes will replicate at a pace of 8 2/3 hours. I will have after a week 4,194,304 total chromosomes of each type since it is pairs replicating. 96,468,992 is the total sum of all chromosomes after the replication. I have extracted the DNA from myself and I am going to undergo surgery for a microarray analysis and then another surgery for any heath problems. I will then after being treated for organ failure like stomach ulcers and heart problems and normal breathing but low O2 in the blood have strong afterpains after the anesthesia wears off. With those afterpains I will either have to brave them and do my eukaryotic cell from scratch as normal or take a strong pain reliever(which is also known as an anesthetic). The problem with that is that some pain relievers make you sleepy and I can't be sleepier than normal for this just to have less pain. Now This doesn't have to do with my PCR question. If you could answer my PCR question that would be nice.
I have heard that with glucose you get 2 ATP from glycolysis and 1 NADH(which is = 3 ATP), 2 NADH from pyruvate -> acetyl CoA, 6 NADH and 2 FADH2( each of which is = 2 ATP) from the krebs cycle so that from glucose you get 38 ATP. I have also heard that an 18 carbon fatty acid gives you 146 ATP. I have not heard of ATP numbers for amino acids(which can enter in glycolysis, pyruvate -> acetyl CoA, or the Krebs Cycle) How do fatty acids give you more ATP than glucose? Does forming nucleotides from Glucose 6 Phosphate give you ATP? What about Gluconeogenesis? Does that have an investment phase and a payoff phase like glycolysis does? Does forming fats and phospholipids from acetyl CoA give you ATP? Does forming amino acids from intermediates in the Krebs cycle give you ATP? This is all relevant to my cell.
