Please i need help concerning how to prepare two-fold serial dilutions of my Antimicrobial agents for Minimum inhibitory concentration (MIC) as follows: I have 500mg/ml (0.5g in 10ml) stock solution and i want to prepare serial dilutions with the following concentrations from it : 250mg/ml, 125mg/ml, 62.5mg/ml and 31.25mg/ml Thanks for your help!
This would be pretty straight forward, and is done quite frequently in the laboratory. The answer would depend on how much of each dilution you require. Assuming that a minimum of 5 mL of each dilution 250 mg/mL to 31.25 mg/mL is desired, you would prepare them as follows: (250 mg/mL) -Mix 5 mL of the 500 mg/mL stock solution with 5 mL of water and mix thoroughly. (125 mg/mL) -Mix 5 mL of the 250 mg/mL stock solution with 5 mL of water and mix thoroughly. (62.5 mg/mL) -Mix 5 mL of the 125 mg/mL stock solution with 5 mL of water and mix thoroughly. (31.25 mg/mL) -Mix 5 mL of the 62.5 mg/mL stock solution with 5 mL of water and mix thoroughly. This procedure would leave you with 5 mL of the 500 mg/mL stock through 62.5 mg/mL dilution, and 10 mL of the 31.25 mg/mL.
