Hello all. A question concerning plant cell cultures, you know, the ones in the nutrient media in the bioreactor. How do these work? I read a little but and i think I get it but still unsure. Also, how easy is it to have certain cells of plants (or animals) create a certain substance, such as a chemical or pharmaceutical? For example, a culture of cannabis cells producing THC oils would be a neat and interesting alternative to growing... Just out of curiosity! any thoughts would be appreciated.
Are you talking about: Sequential Bioreactors? Multiphase Bioreactors? Solid Waste (MSW) Bioreactors? Membrane Bioreactors? Tetrahydrocannabinol Extract: Add 50g grass/liter petroleum ether or benzene; soak twelve hours with occasional shaking; filter and extract petroleum ether three times with a solution containing 5% NaOH and 5% Na2SO3. Acidify the aqueous extracts with cold dilute sulfuric acid and extract with ether or chloroform which is dried, evaporated in a vacuum to yield the cannabidiolic acid. THC and cannabidiol remain in the petrolium ether which can be dried and evaporated in a vacuum and the residue added to the grass. THC acid is converted to THC by boiling in benzene for seven hours. Or just smoke it... heh heh