S.A.M.
11-04-07, 12:39 AM
Movie link (http://www.jbc.org/content/vol0/issue2007/images/data/M706127200/DC1/Supermouse_Video_-_Official_(Windows).wmv)
Phosphoenolpyruvate carboxykinase (PEPCK-C) is an enzyme that is involved in gluconeogenesis in the liver and kidney cortex and in glyceroneogenesis in the liver and white and brown adipose tissue. The enzyme is also present in a wide variety of mammalian tissues such as the small intestine, colon, mammary gland, adrenal gland, lung, and muscle. However, its function in these tissues remains unclear.
http://www.jbc.org/content/vol282/issue45/images/small/zbc0450719460001.gif
PEPCK-Cmus mice contain less body fat than wild-type mice.
Parvin Hakimi and colleagues overexpressed the gene for PEPCK-C in the skeletal muscle of transgenic mice to assess the metabolic and physiological consequences. Surprisingly, they discovered the transgenic mice were 7 times more active than control mice.
The mice were able to exercise on a treadmill for 2-6 km, as compared to 0.2 km for wild-type mice, and they ran twice as fast as control mice. This greatly enhanced exercise capacity was accompanied by a large increase in mitochondria and triglyceride content in the skeletal muscle.
The transgenics were long-lived and retained their enhanced exercise capacity, as well as their fecundity, into murine old age. The mice overexpressing the gene for PEPCK-C also had very little body fat, despite eating 60% more than control mice.
The authors conclude that overexpression of PEPCK-C repatterns energy metabolism and leads to greater longevity.
http://www.jbc.org/cgi/content/abstract/282/45/32844
Phosphoenolpyruvate carboxykinase (PEPCK-C) is an enzyme that is involved in gluconeogenesis in the liver and kidney cortex and in glyceroneogenesis in the liver and white and brown adipose tissue. The enzyme is also present in a wide variety of mammalian tissues such as the small intestine, colon, mammary gland, adrenal gland, lung, and muscle. However, its function in these tissues remains unclear.
http://www.jbc.org/content/vol282/issue45/images/small/zbc0450719460001.gif
PEPCK-Cmus mice contain less body fat than wild-type mice.
Parvin Hakimi and colleagues overexpressed the gene for PEPCK-C in the skeletal muscle of transgenic mice to assess the metabolic and physiological consequences. Surprisingly, they discovered the transgenic mice were 7 times more active than control mice.
The mice were able to exercise on a treadmill for 2-6 km, as compared to 0.2 km for wild-type mice, and they ran twice as fast as control mice. This greatly enhanced exercise capacity was accompanied by a large increase in mitochondria and triglyceride content in the skeletal muscle.
The transgenics were long-lived and retained their enhanced exercise capacity, as well as their fecundity, into murine old age. The mice overexpressing the gene for PEPCK-C also had very little body fat, despite eating 60% more than control mice.
The authors conclude that overexpression of PEPCK-C repatterns energy metabolism and leads to greater longevity.
http://www.jbc.org/cgi/content/abstract/282/45/32844